Figure 5: Phase-contrast image of spiral, filamentous cells
Cells were confined in donut-shaped microchambers fabricated in agarose containing growth media (TB broth, 0.05% BSA, and 20 g/mL cephalexin). An agarose slab (0.5% w/v) or a thin PDMS film (150 um thick) was placed on top of the suspension of cells to provide the ceiling for the chambers; cells were grown for 1-3 h at 33 C. Inset shows magnification of the filamentous cells. The microchambers used in this experiment had the following dimensions: diameter of the inner circle = 4 um, diameter of the outer circle = 13 um, and height = 2 um.