Figure 5: Detecting HIV Ab in human sera using POCKET immunoassay
(A) Schematic diagram of a microfluidic device that detects anti-HIV-1 antibodies. The HIV Env antigen is patterned onto a polystyrene surface, and PDMS microchannels are fabricated on top. A sequence of reagents is added to the microchannels using pressure-driven flow: blocking buffer, human serum sample, gold-labeled anti-human IgG, and the silver enhancement solution. (B) Photographs of the detection areas with reduced silver films. (C) Apparent absorbance values from an immunoassay detecting different dilutions of sera from HIV positive patients and control patients.