Figure 2: A charge ladder of bovine carbonic anhydrase II.
Charge ladder of BCAII formed by partial acetylation of lysine-NH3+ groups and observed by CE. The peak marked by a circle is the neutral marker p-methoxybenzyl alcohol used to monitor electroosmotic flow. The peak marked by a square is the native BCAII. Each following peak represents a mixture of proteins having the same number of acylated amino groups.