Affinity Capillary Electrophoresis

B. F. Shaw, G. F. Schneider, H. Arthanari, M. Narovlyansky, D. T. Moustakas, A. Durazo, G. Wagner, and G. M. Whitesides. 2011. “Complexes of Native Ubiquitin and Dodecyl Sulfate Illustrate the Nature of Hydrophobic and Electrostatic Interactions in the Binding of Proteins and Surfactants.” J. Amer. Chem. Soc., 133, Pp. 17681-17695.Abstract
A. Lee and G. M. Whitesides. 2010. “Analysis of Inorganic Polyphosphates by Capillary Gel Electrophoresis.” Anal. Chem., 82, Pp. 6838-6846.Abstract
PMC# PMC2926810
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G. F. Schneider, B. F. Shaw, A. Lee, E. Carillho, and G. M. Whitesides. 2008. “Pathway for Unfolding of Ubiquitin in SDS, Studied by Capillary Electrophoresis.” J. Amer. Chem. Soc., 130, Pp. 17384-17393.Abstract
PMCID: PMC2688835
K. L. Gudiksen, A. R. Urbach, I. Gitlin, J. Yang, J.A. Vasquez, C.E. Costello, and G. M. Whitesides. 2004. “The Influence of the Zn(II) Cofactor on the Refolding of Bovine Carbonic Anhydrase after Denaturation with Sodium Dodecyl Sulfate.” Analytical Chemistry, 76, Pp. 7151-7161. PDF
X. Chen, H. Wu, C. Mao, and G. M. Whitesides. 2002. “A Prototype 2-D Capillary Electrophoresis System Fabricated in Poly(dimethylsiloxane).” Analytical Chemistry, 74, Pp. 1772-1778. PDF
M. L. Chabinyc, D. T. Chiu, J. C. McDonald, A. D. Stroock, J. F. Christian, A. M. Karger, and G. M. Whitesides. 2001. “An Integrated Fluorescence Detection System in Poly(dimethylsiloxane) for Microfluidic Applications.” Analytical Chemistry, 73, Pp. 4491-4498. PDF
A. D. Stroock, M. Weck, D. T. Chiu, W.T.S. Huck, P. J. A. Kenis, R. F. Ismagilov, and G. M. Whitesides. 2000. “Patterning Electroosmotic Flow with Patterned Surface Charge.” Physical Review Letters, 84, Pp. 3314-3317; Erratum in Phys. Rev. Lett., 2001, 86, 6050. PDF
J. A. Caravella, J. D. Carbeck, D. C. Duffy, G. M. Whitesides, and B. Tidor. 1999. “Long-Range Electrostatic Contributions to Protein-Ligand Binding Estimated Using Protein Charge Ladders, Affinity Capillary Electrophoresis, and Continuum Electrostatic Theory.” J. Am. Chem. Soc., 121, Pp. 4340-4347. PDF
I. J. Colton, J. D. Carbeck, J. Rao, and G. M. Whitesides. 1998. “Affinity capillary electrophoresis: A physical-organic tool for studying interactions in biomolecular recognition.” Electrophoresis, 19, Pp. 367-382. PDF
J. Gao, M. Mrksich, M. Mammen, and G. M. Whitesides. 1998. “Affinity Capillary Electrophoresis: Using Capillary Electrophoresis to Study the Interactions of Proteins with Ligands.” In High Performance Capillary Electrophoresis: Theory, Techniques, and Applications, edited by M. G. Khaledi, 29th ed., 146: Pp. 947-972. New York: John Wiley & Sons, Inc. PDF
J. D. Carbeck, J. C. Severs, J. Gao, Q. Wu, R. D. Smith, and G. M. Whitesides. 1998. “Correlation Between the Charge of Proteins in Solution and in the Gas Phase Investigated by Protein Charge Ladders, Capillary Electrophoresis, and Electrospray Ionization Mass Spectrometry.” J. Phys. Chem. B, 102, Pp. 10596-10601. PDF
J. D. Carbeck, I. J. Colton, J. Gao, and G. M. Whitesides. 1998. “Protein Charge Ladders, Capillary Electrophoresis, and the Role of Electrostatics in Biomolecular Recognition.” Accounts of Chemical Research, 31, Pp. 343-350. PDF
M. Mammen, I. J. Colton, J. D. Carbeck, R. Bradley, and G. M. Whitesides. 1997. “Representing Primary Electrophoretic Data in the 1/Time Domain: Comparison to Representations in the Time Domain.” Analytical Chemistry, 69, Pp. 2165-2170. PDF
J. Rao and G. M. Whitesides. 1997. “Tight Binding of a Dimeric Derivative of Vancomycin with Dimeric L-Lys-D-Ala-D-Ala.” J. Am. Chem. Soc., 119, Pp. 10286-10290. PDF
M. Mammen, J. D. Carbeck, E.E. Simanek, and G. M. Whitesides. 1997. “Treating Electrostatic Shielding at the Surface of Silica as Discrete Siloxide•Cation Interactions.” J. Am. Chem. Soc., 119, Pp. 3469-3476. PDF
J. Rao, I. J. Colton, and G. M. Whitesides. 1997. “Using Capillary Electrophoresis to Study the Electrostatic Interactions Involved in the Association of D-Ala-D-Ala with Vancomycin.” J. Am. Chem. Soc., 119, Pp. 9336-9340. PDF
J. Gao, M. Mammen, and G. M. Whitesides. 1996. “Evaluating Electrostatic Contributions to Binding with the Use of Protein Charge Ladders.” Science, 272, Pp. 535-537. PDF
Y-H. Chu, L. Z. Avila, J. Gao, and G. M. Whitesides. 1995. “Affinity Capillary Electrophoresis.” Acc. Chem. Res., 28, Pp. 461-468. PDF
M. Mammen, F. A. Gomez, and G. M. Whitesides. 1995. “Determination of the Binding of Ligands Containing the N-2,4-Dinitrophenyl Group to Bivalent Monoclonal Rat Anti-DNP Antibody Using Affinity Capillary Electrophoresis.” Anal. Chem., 67, Pp. 3526-3535. PDF
J. Gao, M. Mrksich, F. A. Gomez, and G. M. Whitesides. 1995. “Using Capillary Electrophoresis to Follow the Acetylation of the Amino Groups of Insulin and to Estimate Their Basicities.” Anal. Chem., 67, Pp. 3093-3100. PDF